Update of the simplified criteria for autoimmune hepatitis: evaluation of the methodology for immunoserological testing

Autoantibodies are a hallmark of autoimmune hepatitis and are traditionally tested for by immunofluorescence assays on rodent tissue sections. Herein, we demonstrate that both HEp-2 cells as substrate for ANA IFT and ELISA-based testing are potentially reliable alternatives for autoantibody assessment in autoimmune hepatitis. We propose the implementation of these testing methods into the simplified criteria for the diagnosis of autoimmune hepatitis.